full length recombinant human gfap protein  (Absolute Biotech Inc)

Journal: Cell and Tissue Research

Article Title: Neural crest stem cells protect spinal cord neurons from excitotoxic damage and inhibit glial activation by secretion of brain-derived neurotrophic factor

doi: 10.1007/s00441-018-2808-z

Figure Lengend Snippet: Experimental groups and number of SCSCs ( n )

Article Snippet: Anti-GFAP , Full-length human recombinant GFAP expressed in bacteria , Abcam Cambridge UK, ab7260, AB_305808, rabbit polyclonal , 1:2000.

Techniques: TUNEL Assay, Migration, Enzyme-linked Immunosorbent Assay

Journal: Cell and Tissue Research

Article Title: Neural crest stem cells protect spinal cord neurons from excitotoxic damage and inhibit glial activation by secretion of brain-derived neurotrophic factor

doi: 10.1007/s00441-018-2808-z

Figure Lengend Snippet: a – e Micrographs obtained through the gray matter of SCSCs ( a no-NMDA, b NMDA, c NMDA + NCSC, d NMDA + IL1RA) 10 days after the onset of the experiments using confocal microscopy after staining against GFAP. In order to examine the effect of NCSCs on astroglial activation, non-ramified GFAP-positive astrocytes were counted within the gray matter. Arrows denote non-ramified activated astrocytes, while arrowheads denote astrocytes in a resting state. Graph ( e ) shows the number of non-ramified GFAP positive astrocytes within the gray matter of SCSCs in two different timepoints (statistical analysis was performed by ANOVA followed by Bonferroni correction; solid bars indicate means and error bars denote SEM, p ≤ 0.05)

Article Snippet: Anti-GFAP , Full-length human recombinant GFAP expressed in bacteria , Abcam Cambridge UK, ab7260, AB_305808, rabbit polyclonal , 1:2000.

Techniques: Confocal Microscopy, Staining, Activation Assay

Journal: Cell and Tissue Research

Article Title: Neural crest stem cells protect spinal cord neurons from excitotoxic damage and inhibit glial activation by secretion of brain-derived neurotrophic factor

doi: 10.1007/s00441-018-2808-z

Figure Lengend Snippet: a – j Images of SCSCs maintained for 6 days in vitro after culture preparation and immediate application of NCSCs. Images of unsectioned cultures and neurospheres after light and confocal microscopy ( a – c ): black arrowheads in image of light microscopy ( a ) show neurospheres of NCSCs with no signs of migration. Confocal laser scanning images ( b ) and ( c ) show NCSCs marked with eGFP (green) and NeuN (DyLight-549, red) after immunohistochemistry. NCSCs migrated on top of SCSCs ( c ) but remained in spheres when there was no contact with the cultures ( b ). Images of longitudinal sections through SCSCs and NCSCs ( e , f , g , h ). Diagram ( d ) shows how the cultures were sectioned longitudinally. NCSCs were applied on SCSCs directly after culture preparation in order to avoid scar formation that occurs on top of the cultures and maintained for 6 days in vitro. Images ( g ) and ( h ) represent parts of ( e ) and ( f ) in a higher magnification indicated by dashed lines. The majority of NCSCs migrated on the surface of the culture and some NCSC appeared to migrate through the culture (white arrows). The spheres stained against GFAP showed signs of GFAP immunoreactivity in the edges (white arrowheads in h ) but no signs of co-localization between GFAP and eGFP autofluorescence were observed, suggesting astrocytic migration from SCSCs through the neurospheres. Staining of longitudinal sections against SOX2 and Krox20 ( i , j ). Few cells showed colocalization with SOX2 (small arrows in i ) and Krox20 (large arrows in j ), markers of undifferentiated neural crest stem cells. The small boxes in the upper right-hand corner of ( i ) and ( j ) represent areas of co-localization between eGFP and SOX2 or Krox20, respectively, in higher magnification

Article Snippet: Anti-GFAP , Full-length human recombinant GFAP expressed in bacteria , Abcam Cambridge UK, ab7260, AB_305808, rabbit polyclonal , 1:2000.

Techniques: In Vitro, Confocal Microscopy, Light Microscopy, Migration, Immunohistochemistry, Staining